International Mass Spectrometry Imaging Society Conference III

1- Exploring New Frontiers in Mass Spectrometry Imaging Technology

Chairs: Robert Winkler (PI/Professor Analytical Platform Development,

Advanced Genomics Unit, Cinvestav) and

Łukasz Migas (Researcher MSI and IMS, at Delft University of Technology)

Workshop Description

The workshop aims to motivate and facilitate the exchange of ideas,

challenges and advances in MSI instrumentation and fundamental methods.

A brief introduction will frame the discussion. IMSIS3 participants are invited

to present their ideas in a short (3 min) talk (with/without slides) for

discussion.

CALL

Participants who want to present their project or idea – especially poster

presenters – please contact Robert (robert.winkler@cinvestav.mx).

Topics

• Novel ion sources and desorption techniques

• Ambient ionization/ in vivo analyses/ native tissue MSI

• “Crazy ideas”: Imaging VOCs, whole plants, etc.

• Custom instrumentation and robotics

• FOSS (Free and Open-Source Software) for instrument control

• High-throughput imaging for machine learning/AI

2- Sample Preparation: Mammalian and Clinical Samples

Chairs: Erin H Seeley (Director, Mass Spectrometry Imaging Core Facility,

MD Anderson) and

Michael Becker (Principal Scientist- Mass Spec Imaging and Drug

Metabolism, Boehringer Ingelheim)

Workshop Description

The workshops will consist of the presentation of a few slides that are meant

to trigger discussion from participants. This should be a very interactive

session.

CALL

Participants are encouraged to submit questions to

Erin (EHSeeley@mdanderson.org) or

Michael (michael_4.becker@boehringer-ingelheim.com)

in advance of the session so that the topics can be included in the

workshop.

Topics

• Sample preparation considerations

• Planning a pilot study

• Minimizing Sampling Bias

• Trade-offs between resolution, time, sensitivity, and data size

• Sample cohort size requirements (animal, human, diagnostics)

• MSI “buzz words”

• Working with continuous raster sources

1- From Raw Data to Reliable Insights: MSI Data Processing Essentials

Chairs: Łukasz Migas (Researcher MSI and IMS, at Delft University of

Technology) and

Robert Winkler (PI/Professor Analytical Platform Development, Advanced

Genomics Unit, Cinvestav)

Workshop Description

The workshop covers MSI data processing basics, possible pitfalls, as well

as multi-omics data integration, data sharing and maschine learning.

IMSIS3 participants are invited to present their ideas in a short (3 min) talk

(with/without slides) for discussion.

CALL

Participants who want to present their project or idea – especially poster

presenters – should contact Łukasz (L.G.Migas@tudelft.nl).

Topics

• Brief overview of data processing steps for reliable data science

• Mass alignment & calibration – correcting drift for reproducible results.

• Quality control – metrics, outlier detection, and automated checks.

• Batch correction – reducing technical variability across slides.

• Machine learning – clustering, classification, and interpretability.

• Multimodal integration – co-registration and omics data fusion.

• Data standards – imzML, FAIR principles, and open repositories.

2- Sample Preparation: Non-Mammalian and Environmental Samples

Chairs: Laura Sanchez (Professor of Chemistry & Biochemistry, University

of California Santa Cruz) and

Dušan Veličković (Chemist and Rhizosphere function team lead,

Environmental Molecular Sciences Division, PNNL)

Workshop Description

This workshop will focus on sample preparation considerations for non-

mammalian samples such as bacteria, fungi, mixed co-cultures, soils,

plants, rhizosphere samples. We will specifically focus on examples from

our own labs and the trials and tribulations of preparing non-tissues and

environmental samples. We will close with analyte specific sample

modifications including on-sample chemical derivatization and reactive

matrices.

Timing

10:30-10:50 Preparation of microbial samples

10:50-11:00 Q&A

11:00-11:20 Preparation of soil, plant, and rhizosphere samples

11:20-11:30 Q&A

11:30-11:50 Preparation considerations for specific analytes: on-sample

chemical derivatization and reactive matrices

11:50-Noon Q&A

“Analyses on the edge of our comfort zone: NanoSIMS studies of soil organic matter, microbial interactions and viruses”

1:30pm to 1:45pm - Examining bacterial mechanisms of biocontrol towards fungal pathogens with multimodal spatial mass spectrometry - Kevin Zemaitis

1:45pm to 2:00pm - Optimising plant cell analysis using Water Cluster SIMS at Cryogenic temperatures - Naoko Sano

2:00pm to 2:15pm - Optimized Sample Preparation Strategies for MALDI-MSI of nematode Caenorhabditis elegans - Tian (Autumn) Qiu

2:15pm to 2:30pm - Mass Spectrometry Imaging of the Gut Microbiome -Samarth Ganjoo

“The chemistry of development: Uncovering small molecule regulators of root growth using DESI-MSI"

3:30pm to 3:45pm - Spatial Mapping of Isomeric Inflammation-Mediating Biomolecules with Nano-DESI Mass Spectrometry Imaging - Syeda Nazifa Wali

3:45pm to 4:00pm - Mapping Spatial Lipid Distribution Across Skin Layers and Associated Pathways in Human Skin and Skin Equivalents by MALDI FTICR MSI - Samuele Zoratto

4:00pm to 4:15 - Spatial Lipidomic Profiling Reveals Tumor Microenvironment–Driven Metabolic Plasticity in Rabbit VX2 Models - Mohammad Khavandi

“Mass spectrometry in Space and Prospects for Spaceflight IMS”

From Mars to the icy moon Titan, mass spectrometry is unlocking the secrets of planetary chemistry, with instruments like MOMA on ExoMars and DraMS on Dragonfly pushing the boundaries of in situ exploration. The pioneering spaceflight adaptation of established laboratory techniques in these instruments seeks to detect and characterize complex organics with unprecedented sensitivity, bringing us closer to answering the question of whether life ever emerged elsewhere and how it may have originated on Earth. Emerging innovations within these systems also pave the way for imaging mass spectrometry in space in the not too distant future.

Spatial Biology and Mass Spectrometry: Imaging Across Omics Layers

9:00am to 9:15am - Spatial multi-omics study reveals macrophage driven inflammation and copper dyshomeostasis at heart of delta-sacroglycanopathy model - Maiko Okamura

9:15am to 9:30am - FluoMALDI Microscopy: A Multimodal Imaging Approach for Enhanced Spatial Molecular Discovery with Fluorescent Dyes, Genetic, and Immunofluorescent Labeling - Taehun Hahm

9:30am to 9:45am - Comprehensive same-sample spatial multiomics of drugs, metabolites, mRNAs, and proteins in 2- and 3-dimensions using photocleavable mass-tagged affinity probes - Mark Lim

9:45am to 10:00am - Super-resolved Multimodal Metabolic Barcoding Reveals Phenotype-Specific and Disease-Linked Programs in Tauopathy Mouse Brain - Efe Ozturk

“Towards molecular volume imaging with sub-cellular resolution”

10:30am to 10:45am - Transmission-mode MALDI-2-MSI coupled with In-Source Fluorescence Microscopy for Spatial Biology at Cellular and Sub-Cellular Resolution - Jens Soltwisch

10:45am to 11:00am - Gel-assisted mass spectrometry imaging enables sub-micrometer spatial lipidomics - Yat Ho Chan

11:00am to 11:15am - DESI - High mass and Image resolution at speed - on a bench top mass spectrometer. - Mark Towers

11:15am to 11:30am - Sub-micron 3D-Cryo MSI Reveals Metabolic Variations in Single Cells Using Water and C₆₀ Cluster SIMS - Hua Tian

“Expansion imaging mass spectrometry: high spatial resolution lipid analysis using superabsorbent hydrogels.”

1:30pm to 1:45pm - Multimodal Metabolomic Profiling Identifies Serum Biomarkers and Spatially Resolved Pathways Underlying Endometriosis Pathophysiology and Diagnostics - Sisi Deng

1:45pm to 2:00pm - Evaluation of PEN membrane as a substrate for MALDI MSI in the context of multi-modal workflows - Hugo Delattre

2:00pm to 2:15pm - From serum to tissue: combining multi-omics with MALDI imaging to uncover metabolic maps of pancreatic cancer - Carolin Schneider

2:15pm to 2:30pm - Guided Super-Resolution MALDI-IHC Imaging Reveals Cell-Level Multiomic Profiles in Alzheimer’s Disease Mouse Brain - Leonardo Garcia Dettori

2:30pm to 2:45pm - Integrated Spatial Multiomics Platform to Maximize Tissue-based Discovery in Prostate Cancer - Kristine Glunde

2:45pm to 3:00pm - Novel Multi-Modal MALDI MSI approach Reveals Spatial Immune and Metabolic Reprogramming in BPM31510 Treated Tumors. - Michael Kiebish

 “Refining the role of the host lipid response to pulmonary infection across multiple model systems”

4:00pm to 4:15pm - Lipid and neurotransmitter spatial distribution in transgenic rat model of Alzheimer's disease using MALDI MS imaging - Tong Shen

4:15pm to 4:30pm - A Cross-Sectional Analysis of Lipidomic and Metabolomic Changes due to Organophosphate Nerve Agent Poisoning in a Humanized Mouse Model - Caitlin Tressler

4:30pm to 4:45pm - A new spatial multiomics and multimodal workflow for deeper biological sample analysis in human pancreatic cancer. - Mélanie MOREAU

5:00pm to 5:15pm - Quantitative Mass Spectrometry Imaging of Intact Proteins in Tissues - Mushfeqa Iqfath

5:15pm to 5:30pm - Spatial proteomics and metabolomics of cervical cancer tissues using Imaging Mass Spectrometry - Aastha Baliyan

5:30pm to 5:45pm - Investigating the complex cellular and molecular compositions of lung tissue using multiomics approach - Brittney Gorman

5:45pm to 6:00pm - In situ Tryptic Digestion of Gastric Cancer to Uncover Molecular Differences Linked to Clinically Relevant Parameters - Kristina Schwamborn

“Sample Preparation—A Key to Bioactive Metabolite Mapping in Mass Spectrometry Imaging”

9:00am to 9:15am - Integrated Charged Absorbing Module Tags for MALDI MSI of Steroids in Tissue - Rachel Pryce

9:15am - 9:30am - Rationally Designed Aldehyde-based Reactive Matrices for the Sensitive Detection of Primary Amines and Hydroxyls by MALDI Mass Spectrometry Imaging - Ibrahim Kaya

9:30am - 9:45am - On tissue mapping of rapamycin by MALDI MSI using potassium adducts - Tialfi Bergamin de Castro

9:45am - 10:00am - Expansion Mass Spectrometry Imaging for Subcellular Metabolomics - Laura Sanchez

“Bioinformatics pipeline for reproducible and scalable MALDI imaging workflow of the future”

10:30am to 10:45am - Teardrop: Unsupervised Co-registration of H&E microscopic Images and Mass Spectrometry Images (MSI) with Neural Networks - Sai Srikanth Lakkimsetty

10:45am to 11:00am - Winkler 

11:00am to 11:15am - Targeting neurotransmitter alterations following drug treatment using mass spectrometry imaging and cloud-based data analysis using Weave - Michael Becker

11:15am to 11:30am - Enhanced spatial clustering by applying segmentation to reduced spatial omics data - Bram Heijs

“Molecular and Histological Insights into Pediatric Brain Tumors and

Epilepsy Using DESI-MSI”

9:30am to 9:45am - A unified approach for renal amyloid detection and typing through mass spectrometry imaging and computational pathology - Andrew Smith

9:45am to 10:00am - Spatial Metabolomics of the Human Pathogen Helicobacter pylori: Integrating Chronic In Vivo and Novel In Vitro Models of Infection - Benedikt Geier

10:00am to 10:15am - Mass Spectrometry Imaging Reveals Evidence of Direct Localized Covalent Chemistry in Thermoembolization - Erik Cressman

10:15am to 10:30am - Spatial Lipid Profiling in Myelomeingocele with MALDI MSI and Tandem Mass Spectrometry - Arya Lall

"Stress Signals on Neural Circuit visualized with IMS”

11:00am to 11:15am - Exploring lipidomic, proteomic and histological heterogeneity of a venous thromboembolism (VTE) - Nina Ogrinc

11:15am to 11:30am - Lipid Aging and Localization in Ocular Tissues - Kevin Schey

11:30am to 11:45am - Visualizing the incorporation of deuterium-reinforced polyunsaturated fatty acids (D-PUFAs) in mice retina using MALDI-2-MS Imaging - KLAUS DREISEWERD

11:45am to 12:00pm - Chemotherapy Resistance Investigated by Multimodal Mass Spectrometry Imaging of Osteosarcoma Multicellular Tumour Spheroid Models - Sophie Pearce

“Direct 3D mass spectrometry imaging”

1:30pm to 1:45pm - Development of a compact t-SPESI system for high-sensitivity single-cell mass spectrometry imaging - Yoichi Otsuka

1:45pm to 2:00pm - Integration of Signal Improvement Techniques for IR-MALDESI Mass Spectrometry Imaging - Seth Eisenberg

2:00pm to 2:15pm - Tandem mass spectrometry imaging for next level annotation with spatial correlation of product ions - Ingela Lanekoff

2:15pm to 2:30pm - From Tissues to Single Cells: Direct Proteoform Profiling Using Orbitrap-Based Single Molecule Mass Spectrometry - Pei Su

3:30pm to 3:45pm - Advancing Precision Diagnosis of Cervical Cancer and Precancers Through Multimodal Mass Spectrometry Imaging - Stefania Maneta-Stavrakaki

3:45pm to 4:00pm - Combining On-Tissue Derivatization with Sublimation-Based Matrix Application for High-Resolution MALDI Imaging of Carbonyl-Containing Metabolites - Paul Klessen

4:00pm to 4:15pm - Characterizing Source Configuration in MALDI and MALDESI-Based Mass Spectrometry Imaging Platforms for Diverse Molecular Profiling - Sarah Ashbacher

4:15pm to 4:30pm - Targeted High Spatial Resolution Metabolite Imaging using DESI on Tandem Quadrupole MS - Bindesh Shrestha

4:30pm to 4:45pm - Multimodal phenotyping of circulating human immune cells using MALDI-MS and immunofluorescence imaging - Sebastian Bessler

“Ambient Mass Spectrometry Imaging: Towards Comprehensive Molecular Mapping of Biological Systems”

Ambient mass spectrometry imaging (MSI) has emerged as a powerful tool for spatially resolved molecular analysis of biological systems. This presentation will highlight recent advances in ambient MSI techniques, with a particular focus on nanospray desorption electrospray ionization (nano-DESI) and other liquid extraction-based ionization techniques. Nano-DESI offers the advantage of analyzing samples under ambient conditions with minimal preparation, preserving the native state of the analytes and facilitating high throughput data acquisition. Recent developments in nano-DESI MSI instrumentation and methodologies enable quantitative imaging of a wide range of biomolecules, including lipids, metabolites, and intact proteoforms, in complex biological tissues. The application of MSI in tandem mass spectrometry (MS/MS) mode provides even greater specificity, enabling isomer-selective imaging. These advancements have facilitated a deeper understanding of tissue organization, function, and disease mechanisms. The integration of MSI data with other imaging modalities, such as immunofluorescence, provides a more comprehensive molecular map of biological systems.